https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38372 Wed 01 Sep 2021 12:12:32 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34881 Momordica cochinchinensis Spreng) seed kernels. To improve the method, a solvent evaporation step was added prior to the colorisation reaction to prevent undesired solvent interference during the reaction step. Using this modified protocol for the aescin standard curve and the Gac seed kernel extract eliminated any solvent interference. Thus, this improved protocol is recommended for the quantification of the saponin content of plant extracts irrespective of which extraction solvent is used.]]> Tue 03 Sep 2019 18:09:42 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:17524 2PO₄/Na₂HPO₄ and KH₂PO₄/Na₂HPO₄.2H₂O buffer solutions at pH of 6.7 and 6.9. Buffer solution containing phosphate composition of KH₂PO₄/Na₂HPO₄·2H₂O (pH=7.0) gives relatively stable values for MetHb during the storage and the amount of MetHb samples in the buffer solution retain constant up to 9 days. Therefore, stabilized MetHb blood samples can be prepared using KH₂PO₄/Na₂HPO₄·2H₂O buffer solution (pH=7) with non-ionic detergent and the samples can be stored for several days at 4–8°C.]]> Sat 24 Mar 2018 08:03:53 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:17893 – ion selective electrode. The reaction results in the formation of nitric oxide and thiocyanogen, the latter decomposing to sulfate and hydrogen cyanide in aqueous solution. The rate of consumption of ONSCN depends strongly on the concentration of SCN^– ions and is inhibited by nitric oxide. We have developed a reaction mechanism that comprises three parallel pathways for the decomposition of ONSCN. At high thiocyanate concentrations, two reaction pathways operate including a second order reaction to generate NO and (SCN)₂ and a reversible reaction between ONSCN and SCN^– producing NO and (SCN)₂^–, with the rate limiting step corresponding to the consumption of (SCN)₂^– by reaction with ONSCN. The third reaction pathway, which becomes significant at low thiocyanate concentrations, involves formation of a previously unreported species, ONOSCN, via a reaction between ONSCN and HOSCN, the latter constituting an intermediate in the hydrolysis of (SCN)₂. ONOSCN contributes to the formation of NO via homolysis of the O–NO bond and subsequent dimerization and hydrolysis of OSCN. Fitting the chemical reactions of the model to the experimental measurements, which covered a wide range of reactant concentrations, afforded estimation of all relevant kinetic parameters and provided an excellent match. The reaction mechanism developed in this contribution may be applied to predict the rates of NO formation from ONSCN during the synthesis of azo dyes, the gassing of explosive emulsions, or nitrosation reactions occurring in the human body.]]> Sat 24 Mar 2018 07:56:25 AEDT ]]>